Conservation tillage regulates the assembly, network structure and ecological function of the soil bacterial community in black soils

Traditional tillage represents a serious threat to the stability of soil ecosystems. Understanding response mechanisms microbial community assembly different practices is major topic ecological research. Here, we investigated bacterial structures and in bulk rhizosphere soils soybeans grown under traditional (moldboard plow, MP) two conservation practices, namely, no-tillage (NT) ridge (RT), using high-throughput sequencing methods. Compared with MP, NT RT increased relative abundances nitrifying bacteria Nitrosospira sp. nitrogen-fixing Mesorhizobium sp., Bradyrhizobium Burkholderia but decreased abundance carbon-degrading bacteria, especially Blastococcus Streptomyces Sphingomonas The altered functional were mostly affiliated biomarkers keystone taxa networks. For results network properties processes, found that habited more stable structure lower homogenizing dispersal value. Soil pH was primary factor regulating both processes three practices. profoundly by changes indicated might contribute carbon sequestration, while stimulating nitrogen fixation nitrification.